Effect of organo-selenium anticancer drugs on nitrite induced methemoglobinemia: A spectroscopic study

Abstract Selenium containing drugs like selenomethionine, selenocystine, selenourea and methylseleninic acid are reported to exhibit potential anticancer effect. However, these may exert adverse effects when used over a prolonged period. Little is known about the interaction of selenium with vital erythroid protein hemoglobin. In this work comparative study organo-selenium hemoglobin heme moiety has been performed using different spectroscopic techniques find out their role on drug induced methemoglobinemia. We found that though have similar binding affinity towards hemoglobin, they differential interactions group. Isothermal calorimetric titration showed lowest (Kd 19.28 ?M) HbA as compared other drugs, selenocystine 7.69 ?M, 4.88 ?M 10.5 ?M at 37 °C respectively). This result also supported by molecular docking study. Methylseleninic was detrimental nitrite methemoglobinemia, hematological disorder caused due excessive conversion Fe2+ Fe3+ in Hence results would help develop better insight mechanism action anticipate toxicity which require further optimization before actual use treatment cancer.